From Lehningher principles of Biochemistry, this is a conceptual diagram of initiation of protein synthesis in a prokaryote:
You can expand and examine the images through this link:
https://liarsfordarwin.files.wordpress.com/2017/05/prokayote_protein_synthesis_initiation.png
This is a conceptual diagram of initiation of protein synthesis in a eukaryote:
You can expand and examine the images through this link:
https://liarsfordarwin.files.wordpress.com/2017/05/eukaryote_protein_synthesis_initiation1.png
For starters, the Shine Dalgarno sequence isn’t normally present in Eukaryotic DNA even for homologous genes, not to mention before the mRNAs are formed, eukaryotic genes homologous to prokaryotic genes have to have their spliceosomal introns spliced out. Next it is readily apparent the initiation complexes and sequence of steps are different. If eukaryotes evolved from prokaryotes or if both evolved from a common ancestor, then how did changes in life critical steps emerge without killing the organism? Clearly there are life critical parts. The prokaryotic initiation is simpler, so how did the more complex eukaryotic system evolve. First notice the different order of the IF-3 and IF-1 factors binding to the E and A location on the 30S subunit vs. the eIF1, eIF3, and eIF1A binding to the E, P, A locations on the 405 subunit.
I could go one and on, but hopefully the reader gets the picture! 🙂